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Structured Review

Promega rna cleavage buffer
Rna Cleavage Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+cleavage+buffer/rna+cleavage+buffer/pm37770519-253-63-80
Average 90 stars, based on 1 article reviews
rna cleavage buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

In Vitro:

Article Title: Recognition and cleavage mechanism of intron-containing pre-tRNA by human TSEN endonuclease complex.
Article Snippet: .. In vitro transcription was carried out with 2μg DNA templates at 37 °C for 16 h. RNA substrates were separated and concentrated through the HiPure RNA Pure Micro Kits (Magen). tRNA intron splicing assay The RNA substrates were heated at 95 °C for 5min, followed by a slow cooling to room temperature to allow for annealing. tRNA cleavage reactions were carried out in RNA cleavage buffer (50mM KCl, 50mM HEPES pH 8.0, 1mM MgCl2, 1mM DTT and 1 unit/mL RNAsin (Promega)). ..

Splicing Assay:

Article Title: Recognition and cleavage mechanism of intron-containing pre-tRNA by human TSEN endonuclease complex.
Article Snippet: .. In vitro transcription was carried out with 2μg DNA templates at 37 °C for 16 h. RNA substrates were separated and concentrated through the HiPure RNA Pure Micro Kits (Magen). tRNA intron splicing assay The RNA substrates were heated at 95 °C for 5min, followed by a slow cooling to room temperature to allow for annealing. tRNA cleavage reactions were carried out in RNA cleavage buffer (50mM KCl, 50mM HEPES pH 8.0, 1mM MgCl2, 1mM DTT and 1 unit/mL RNAsin (Promega)). ..



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